Re: NANFA-L-- Frozen fish embryos?

Henry G. Tomasiewicz (henryt-in-uwm.edu)
Thu, 25 May 2006 08:54:52 -0500

Hi,

Au Contraire, or something like that,it is much harder to freeze down fish eggs
than one would think. The difficulty it is thouht lies in the many different
membranes and structures with vastly different lipid composition, each of which
require a different freezing rate or storage buffer to survive the freezing
process and storage. Several groups have been working on this in zebrafish
for several years, because of the desire to backup all of the mutant lines that
are used. Can freeze zebrafish sperm, but not fertilized embryos. Mouse
embryos can be frozen, but even those require controlled freezing rate. Would
be worth figuring out a way to do this. May want to try figuring out how to
freeze embryo nuclei so they are viable and can be injected back into the
enucleated eggs of a similar species-in-a later date.

HenryT

"The idea is to die young as late as possible" Ashley Montagu

Quoting Mysteryman <bestfish-in-alaweb.com>:

> I'd be willing to bet that freezing fertilized fish eggs would be a very
> simple thing to do, and that it wouldn't be too much trickier to apply
> similar technique to the vivaparous fishes. It would also be very simple
> in most cases to mass-produce those fertilized eggs in any fishfarm
> laboratory using standard mass-culture techniques.
> In other words, this part of the plan should be a relative breeze.
> Dealing with those eggs once thawed would be the hard part.
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